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This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 µg/l to 80 µg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 µg/l for standards and 0,09 µg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
Author | ISO/TC 34 Food products |
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Editor | ISO |
Document type | Standard |
Format | Paper |
Edition | 1 |
ICS | 67.050 : General methods of tests and analysis for food products
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Number of pages | 20 |
Weight(kg.) | 0.1340 |
Year | 2019 |
Country | Switzerland |